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1x neb buffer 4  (New England Biolabs)


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    Structured Review

    New England Biolabs 1x neb buffer 4
    1x Neb Buffer 4, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+neb+buffer+4/NEBuffer+4/bio_rxiv__64898__2026__02__12__705613-217-32-33
    Average 98 stars, based on 1093 article reviews
    1x neb buffer 4 - by Bioz Stars, 2026-10
    98/100 stars

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    Incubation:

    Article Title: Genome-wide single-cell perturbation screens with VIPerturb-seq
    Article Snippet: .. During wash steps, the intracellular antibody pool prepared as described above was incubated with 8 μg of single-stranded DNA binding protein (Promega M3011) per 1 μg of antibody in a solution of 1x NEB buffer 4 (NEB B7004S) for 30 minutes at 37 ° C. After completing the incubation, BSA and PBS were added to a final concentration of 3% BSA in 1x PBS. ..

    Article Title: Phospho-seq: integrated, multi-modal profiling of intracellular protein dynamics in single cells
    Article Snippet: After 5 min, wash buffer #1 (10 mM Tris-HCl, 10 mM NaCl, 3.33 mM MgCl 2 , 1% BSA in H 2 O) was added up to 1 ml and cells were centrifuged for 5 min at 500 × g after which the supernatant was discarded and the cells were resuspended in staining buffer (3% BSA in PBS) for blocking and placed in a tube rotator at RT for 30 min. For cells that received a primary antibody bound by EcoRI single-stranded DNA binding protein (SSB) (Promega: M3011), the SSB binding reaction happened during the blocking step. .. Briefly, 1 μg of antibody was incubated with 8 μg of SSB in a solution of 1x NEB buffer 4 (NEB:B7004S) for 30 min at 37 °C. ..

    Article Title: Phospho-seq: integrated, multi-modal profiling of intracellular protein dynamics in single cells.
    Article Snippet: For cells that received a primary antibody bound by EcoRI singlestranded DNA binding protein (SSB) (Promega: M3011), the SSB binding reaction happened during the blocking step. .. Briefly, 1μg of antibody was incubated with 8μg of SSB in a solution of 1x NEB buffer 4 (NEB:B7004S) for 30min at 37 °C. ..

    Article Title: Iterative, multimodal, and scalable single-cell profiling for discovery and characterization of signaling regulators
    Article Snippet: While in the blocking and hashing step, the primary antibody pool was incubated with single-stranded DNA binding protein (Promega: M3011) to help reduce off-target effects , . .. Briefly, 8 μg of SSB was added per 1 μg of antibody in a solution of 1x NEB buffer 4 (NEB:B7004S) for 30 mins at 37 C. After this incubation, BSA and PBS were added to make a final solution of 3% BSA in 1X PBS. ..

    Binding Assay:

    Article Title: Genome-wide single-cell perturbation screens with VIPerturb-seq
    Article Snippet: .. During wash steps, the intracellular antibody pool prepared as described above was incubated with 8 μg of single-stranded DNA binding protein (Promega M3011) per 1 μg of antibody in a solution of 1x NEB buffer 4 (NEB B7004S) for 30 minutes at 37 ° C. After completing the incubation, BSA and PBS were added to a final concentration of 3% BSA in 1x PBS. ..

    Concentration Assay:

    Article Title: Genome-wide single-cell perturbation screens with VIPerturb-seq
    Article Snippet: .. During wash steps, the intracellular antibody pool prepared as described above was incubated with 8 μg of single-stranded DNA binding protein (Promega M3011) per 1 μg of antibody in a solution of 1x NEB buffer 4 (NEB B7004S) for 30 minutes at 37 ° C. After completing the incubation, BSA and PBS were added to a final concentration of 3% BSA in 1x PBS. ..

    Suspension:

    Article Title: Methods for the Preparation of Large Quantities of Complex Single-Stranded Oligonucleotide Libraries
    Article Snippet: .. The beads are washed twice with 200 µl 1x NEB buffer 4, followed by suspension of beads in 100 µl of enzyme mix containing 50 U BspQI enzyme, 1x NEB buffer 4 and 1x BSA. ..



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